DNA barcoding of mosquitoes collected through a nationwide survey in 2011 and 2012 in Malawi, Southeast Africa.

作者: Yoshihide Maekawa , Dylo Pemba , Justin Kumala , Steve Gowelo , Yukiko Higa

DOI: 10.1016/J.ACTATROPICA.2020.105742

关键词:

摘要: We conducted a nationwide survey of mosquito distribution in Malawi from November 2011 to April 2012, and July September 2012. Using dried specimens adults collected during the survey, we analyzed their cytochrome c oxidase subunit I (COI) gene sequences, prepared specimens, registered genetic information (658 bp) 144 individuals belonging 51 species 10 genera GenBank. obtained information, degree intraspecific variation investigated various morphological perspectives. Moreover, phylogenetic analysis medically important distributed Africa Asia explored geographical differentiation. Results showed that morphologically classified as Culex univittatus complex included individual Cx. perexiguus which, date, have not been reported southern Africa. Furthermore, Mansonia uniformis, Asia, was revealed belong genetically distinct populations, with observed differences samples suggesting they are separate species. The results further suggested ethiopicus is synonym bitaeniorhynchus, but it an independent species; although, this study, only definite difference shape wing scales. Further investigation these species, including larvae, highly recommended.

参考文章(39)
Kiyoyuki Mizusawa, Edward S. Saugstad, Kazuo Tanaka, A Revision of the Adult and Larval Mosquitoes of Japan (Including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae) Contrib. Am. Entomol. Inst.. ,vol. 16, pp. 1- 987 ,(1979)
Tetsuro Ikegami, Shinji Makino, Rift Valley fever virus Uirusu. ,vol. 54, pp. 229- 235 ,(2004) , 10.2222/JSV.54.229
Ralph E. Harbach, Kenneth L. Knight, Taxonomists' glossary of mosquito anatomy Taxonomists' glossary of mosquito anatomy.. ,(1980)
M. T. Gillies, B. De Meillon, The Anophelinae of Africa south of the Sahara (Ethiopian Zoogeographical Region). The Anophelinae of Africa south of the Sahara (Ethiopian Zoogeographical Region).. ,(1968)
R. E. Harbach, The mosquitoes of the subgenus Culex in southwestern Asia and Egypt (Diptera: Culicidae). Contributions of the American Entomological Institute. ,vol. 24, ,(1988)
Lawrence N Kazembe, Immo Kleinschmidt, Timothy H Holtz, Brian L Sharp, Spatial analysis and mapping of malaria risk in Malawi using point-referenced prevalence of infection data. International Journal of Health Geographics. ,vol. 5, pp. 41- 41 ,(2006) , 10.1186/1476-072X-5-41
Julie A. Scott, William G. Brogdon, Frank H. Collins, Identification of Single Specimens of the Anopheles Gambiae Complex by the Polymerase Chain Reaction American Journal of Tropical Medicine and Hygiene. ,vol. 49, pp. 520- 529 ,(1993) , 10.4269/AJTMH.1993.49.520
C. van den Bosch, G. Lloyd, Chikungunya fever as a risk factor for endemic Burkitt's lymphoma in Malawi. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 94, pp. 704- 705 ,(2000) , 10.1016/S0035-9203(00)90240-2