A decade of G3P[8] and G9P[8] rotaviruses in Brazil: epidemiology and evolutionary analyses.

作者: Mariela Martínez Gómez , Filipe Anibal Carvalho-Costa , Eduardo de Mello Volotão , Tatiana Lundgren Rose , Marcelle Figueira Marques da Silva

DOI: 10.1016/J.MEEGID.2014.05.016

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摘要: Abstract This study aims to estimate the frequency of group A rotaviruses (RVA) infection with genotypes G3P[8] and G9P[8] in children that suffered from diarrheal disease (DD) between 2001 2011 different Brazilian regions. In addition, genetic diversity RVA strains recovered vaccinated non-vaccinated was assessed. Laboratory-based surveillance included 15,115 cases DD, detected by enzyme immune-assay and/or polyacrylamide gel electrophoresis 3357 (22%) samples. genotyped semi-nested RT-PCR among RVA-positive samples, 100 (2.9%) were G3 (63 G3P[8], 32 G3P not typed [NT], 5 G3P[6]) 378 (16.2%) G9 (318 G9P[8], 59 G9P[NT], 1 G9P[6]). From positive 16 12, respectively, obtained aged 4–48 months monovalent vaccine (Rotarix®, RV1). Phylogenetic analyses VP7 VP8 ∗ encoding genes performed for 26 48 strains. phylogenetic analysis revealed all analyzed belonged P[8] lineage III, whereas RV1 belongs P[8]-I lineage. G3-lineage III G9-lineage III. The comparison antigenic epitopes regions strain several amino acid changes. However, no particular differences before after introduction observed, or children. Complete genome characterization four seven a typical conserved human Wa-like genomic constellation. Changes Brazil seemed be related Brazil.

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