Testicular Stem Cells Express Follicle-Stimulating Hormone Receptors and Are Directly Modulated by FSH.

摘要: Testicular spermatogonial stem cells (SSCs) are a heterogeneous population of cells, and definitive marker for the most primitive subset that undergoes asymmetric cell division remains to be identified. A novel subpopulation pluripotent, very small embryonic-like (VSELs) has been reported in both human mouse testes. Follicle-stimulating hormone (FSH) receptors (FSHRs) expressed on Sertoli testis granulosa ovary, but recently FSHRs VSELs ovaries, bone marrow, cord blood. The present study was aimed investigate whether also testicular (VSELs SSCs) their possible modulation by FSH using intact chemoablated (25 mg/kg busulfan) mice. Chemoablated better model biology since quiescent survive along with tubules. Proliferating nuclear antigen-positive, small-sized presumed were clearly visualized, flow cytometry analysis revealed an increase LIN-/CD45-/SCA-1+ from 0.045±0.008% 0.1±0.03% total after treatment. Very expressing octamer-binding transcription factor 4 (OCT-4) SSCs cytoplasmic OCT-4 detected. (Oct-4A, Sca-1, Nanog), (Oct-4), proliferation (Pcna) specific transcripts upregulated Stem FSHR stimulated FSH, Fshr3 predominant transcript maximally modulated FSH. Nuclear SCA-1 (stem antigen 1) positive testis, stimulates them undergo including self-renewal give rise SSCs, which turn proliferate rapidly clonal expansion further differentiation.