H2O2 sensitivity of afferent splanchnic C fiber units in vitro

摘要: 1. Single-unit impulse activity evoked by transient, focal application of hydrogen peroxide (H2O2) to identified visceral receptive fields has been characterized in an vitro rat splanchnic nerve-mesentery preparation. In addition H2O2 responsiveness, units were terms sensitivity mechanical stimuli, warming, and bradykinin. 2. Mesenteric single afferent C fibers located with the use warm (approximately 45 degrees saline) or search stimuli. After delimitation warm-sensitive and/or mechanosensitive field, tested for responsiveness H2O2. Microliter volumes (usually 1 microliter) (88-880 mM) responses 25 42 (60%) fields, additional 10 which was only effective stimulus. 3. Tachyphylaxis repeated stimulation observed interstimulus intervals 2 min) response latencies defined fields. 7. Excitation neurons may be relevant modulation reactive oxygen species production immunocompetent cells, because sensory neuropeptides contained these are known influence respiratory burst macrophages neutrophils.