A new conjugated linoleic acid isomer, 7 trans, 9 cis-octadecadienoic acid, in cow milk, cheese, beef and human milk and adipose tissue
作者： Martin P. Yurawecz , John A. G. Roach , Najibullah Sehat , Magdi M. Mossoba , John K. G. Kramer
DOI: 10.1007/S11745-998-0273-Z
关键词:
摘要: The identity of a previously unrecognized conjugated linoleic acid (CLA) isomer, 7 trans, 9 cis-octadecadienoic (18∶2) was confirmed in milk, cheese, beef, human and adipose tissue. cis-18∶2 isomer resolved chromatographically as the methyl ester by silver ion-high-performance liquid chromatography (Ag+-HPLC); it eluted after major cis, 11 trans-18∶2 (rumenic acid) natural products analyzed. In biological matrices in-vestigated Ag+-HPLC, peak generally due to most abundant minor CLA ranging concentration from 3 16% total CLA. By gas (GC) with long polar capillary columns, shown elute near leading edge peak, while 4,4-dimethyloxazoline (DMOX) derivative permitted partial resolution these two isomers. DMOX this new analyzed chromatography-electron ionization mass spectrometry (GC-EIMS). double bond positions were at Δ7 Δ9 indicated characteristic spectral fragment ions m/z 168, 180, 194, 206, their allylic cleavages 154 234. cis/trans double-bond configuration established GC-direct deposition-Fourier transform infrared evidenced doublet 988 949 cm−1 absorptions 3020 3002 cm−1. GC-EIMS for examined, comparison similar product obtained treatment mixture 8-hydroxy-and 11-hydroxyoctadec-9 cis enoates BF3, methanol.
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Michael R. Pollard, Frank D. Gunstone, Anthony T. James, Lindsay J. Morris, Desaturation of positional and geometric isomers of monoenoic fatty acids by microsomal preparations from rat liver Lipids. ,vol. 15, pp. 306- 314 ,(1980) , 10.1007/BF02533545
Tianan Jiang, Azlin Mustapha, Dennis A. Savaiano, Improvement of lactose digestion in humans by ingestion of unfermented milk containing Bifidobacterium longum Journal of Dairy Science. ,vol. 79, pp. 750- 757 ,(1996) , 10.3168/JDS.S0022-0302(96)76422-6
F. Lavillonnière, J. C. Martin, P. Bougnoux, J.-L. Sébédio, Analysis of conjugated linoleic acid isomers and content in French cheeses. Journal of the American Oil Chemists' Society. ,vol. 75, pp. 343- 352 ,(1998) , 10.1007/S11746-998-0051-6
Michelle K. McGuire, Yongsoon Park, Rebecca A. Behre, Lisa Y. Harrison, Terry D. Shultz, Mark A. McGuire, Conjugated linoleic acid concentrations of human milk and infant formula Nutrition Research. ,vol. 17, pp. 1277- 1283 ,(1997) , 10.1016/S0271-5317(97)00111-5
J. Y. Zhang, Q. T. Yu, B. N. Liu, Z. H. Huang, Chemical modification in mass spectrometry IV—2-alkenyl-4,4-dimethyloxazolines as derivatives for the double bond location of long-chain olefinic acids Journal of Mass Spectrometry. ,vol. 15, pp. 33- 44 ,(1988) , 10.1002/BMS.1200150106
M. M. Mossoba, R. E. McDonald, J. A. G. Roach, D. D. Fingerhut, M. P. Yurawecz, N. Sehat, Spectral confirmation of trans monounsaturated C18 fatty acid positional isomers Journal of the American Oil Chemists' Society. ,vol. 74, pp. 125- 130 ,(1997) , 10.1007/S11746-997-0156-3
John K. G. Kramer, Vivek Fellner, Michael E. R. Dugan, Frank D. Sauer, Magdi M. Mossoba, Martin P. Yurawecz, Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids Lipids. ,vol. 32, pp. 1219- 1228 ,(1997) , 10.1007/S11745-997-0156-3
P.W. Parodi, Conjugated Octadecadienoic Acids of Milk Fat Journal of Dairy Science. ,vol. 60, pp. 1550- 1553 ,(1977) , 10.3168/JDS.S0022-0302(77)84068-X
J K G Kramer, H W Hulan, A comparison of procedures to determine free fatty acids in rat heart. Journal of Lipid Research. ,vol. 19, pp. 103- 106 ,(1978) , 10.1016/S0022-2275(20)41581-0
F D Sauer, V Fellner, R Kinsman, J K Kramer, H A Jackson, A J Lee, S Chen, Methane output and lactation response in Holstein cattle with monensin or unsaturated fat added to the diet Journal of Animal Science. ,vol. 76, pp. 906- 914 ,(1998) , 10.2527/1998.763906X