Transient expression of an active human interferon-beta in lettuce

摘要: Abstract Using Agrobacterium mediated transient expression method, plant bivalent vector pBI121 containing GUS as a report gene was transformed into lettuce (Lactuca sativa). Through designed orthogonal analysis, intact leaves infiltrated with 200 μM acetosyringone and 0.8 OD600 bacterial suspensions under vacuum for 30 min, then co-cultured at 24 °C 6 ds produced maximum protein of 2.5% TSP 21.39 nmol mg−1 min−1 MU activity, which 19 times the control (1.31 nmol mg−1 min−1 MU). Employed these optimized conditions HuIFN-beta expressed in leaves. Western blot antivirus bioactivity analyses confirmed achieved by agrobacterium infiltration had high biological activity (3.1 × 104 IU/mL). To our knowledge, it is first detailed optimizing study on production biologically active therapeutic proteins lettuce. In summary, can be adopted an efficient, inexpensive small-scaled system production.