作者: C. Löwe , C. Weder
DOI: 10.1002/1521-4095(20021118)14:22<1625::AID-ADMA1625>3.0.CO;2-Q
关键词:
摘要: … 13000 g and resuspended in 1 mL PIPES buffer containing 10 mM taxol. The microtubules … in PIPES) for 2 h at room temperature and dialyzed against tenfold diluted PIPES buffer for 2 h…