3D Porous Chitosan-Alginate Scaffolds: A New Matrix for Studying Prostate Cancer Cell-Lymphocyte Interactions In Vitro
作者： Stephen J. Florczyk , Gang Liu , Forrest M. Kievit , Allison M. Lewis , Jennifer D. Wu
关键词:
摘要: The treatment of castration-resistant prostate cancer (CRPC) remains palliative. Immunotherapy offers a potentially effective therapy for CRPC; however, its advancement into the clinic has been slow, in part because lack representative vitro tumor models that resemble vivo microenvironment studying interactions CRPC cells with immune and other potential therapeutics. This study evaluates use 3D porous chitosan-alginate (CA) scaffolds culturing human (PCa) cell interaction peripheral blood lymphocytes (PBLs) ex vivo. CA Matrigel matrix samples supported spheroid formation over 15 days culture, live fluorescence imaging confocal microscopy using stably transfected PCa 55 days. grown were co-cultured PBLs 2 6 evaluated scanning electron (SEM), immunohistochemistry (IHC), flow cytometry. Both tumors, providing more robust platform subsequent analyses. demonstrates natural polymer as tissue culture model supporting long-term analysis cells, an rapid immunotherapy development.
elsevier.com
sci-hub.se 参考文章(47)
Pedro Romero, Jean-Charles Cerottini, Daniel E. Speiser, Monitoring tumor antigen specific T-cell responses in cancer patients and phase I clinical trials of peptide-based vaccination. Cancer Immunology, Immunotherapy. ,vol. 53, pp. 249- 255 ,(2004) , 10.1007/S00262-003-0473-9
Virginie Dangles, Pierre Validire, Mireille Wertheimer, Sophie Richon, Christophe Bovin, Dominique Zeliszewski, Guy Vallancien, Dominique Bellet, Impact of human bladder cancer cell architecture on autologous T-lymphocyte activation. International Journal of Cancer. ,vol. 98, pp. 51- 56 ,(2002) , 10.1002/IJC.10140
Akira Tasaki, Naoki Yamanaka, Makoto Kubo, Kotaro Matsumoto, Hideo Kuroki, Katsuya Nakamura, Chihiro Nakahara, Hideya Onishi, Hirotaka Kuga, Eishi Baba, Masao Tanaka, Takashi Morisaki, Mitsuo Katano, Three-dimensional two-layer collagen matrix gel culture model for evaluating complex biological functions of monocyte-derived dendritic cells. Journal of Immunological Methods. ,vol. 287, pp. 79- 90 ,(2004) , 10.1016/J.JIM.2004.01.014
Claudia Fischbach, Ruth Chen, Takuya Matsumoto, Tobias Schmelzle, Joan S Brugge, Peter J Polverini, David J Mooney, Engineering tumors with 3D scaffolds. Nature Methods. ,vol. 4, pp. 855- 860 ,(2007) , 10.1038/NMETH1085
Stephen R. Plymate, David Cosman, Jennifer D. Wu, Lily M. Higgins, Kathy Haugk, Alexander Steinle, Prevalent expression of the immunostimulatory MHC class I chain–related molecule is counteracted by shedding in prostate cancer Journal of Clinical Investigation. ,vol. 114, pp. 560- 568 ,(2004) , 10.1172/JCI22206
R. Chignola, A. Schenetti, G. Andrighetto, E. Chiesa, R. Foroni, S. Sartoris, G. Tridente, D. Liberati, Forecasting the growth of multicell tumour spheroids: implications for the dynamic growth of solid tumours. Cell Proliferation. ,vol. 33, pp. 219- 229 ,(2000) , 10.1046/J.1365-2184.2000.00174.X
M. H. Zaman, L. M. Trapani, A. L. Sieminski, D. MacKellar, H. Gong, R. D. Kamm, A. Wells, D. A. Lauffenburger, P. Matsudaira, Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 10889- 10894 ,(2006) , 10.1073/PNAS.0604460103
Jérôme Martin, Céline Bossard, Arnaud Boureille, Régis Josien, Differential regulation of IL-22BP in Crohn’s disease versus ulcerative colitis Journal of Translational Medicine. ,vol. 9, pp. 11- ,(2011) , 10.1186/1479-5876-9-S2-P11
H WANG, D YANG, W XU, Y WANG, Z RUAN, T ZHAO, J HAN, Y WU, Tumor-derived soluble MICs impair CD3+CD56+ NKT-like cell cytotoxicity in cancer patients Immunology Letters. ,vol. 120, pp. 65- 71 ,(2008) , 10.1016/J.IMLET.2008.07.001
Brian M. Olson, Douglas G. McNeel, CD8+ T cells specific for the androgen receptor are common in patients with prostate cancer and are able to lyse prostate tumor cells Cancer Immunology, Immunotherapy. ,vol. 60, pp. 781- 792 ,(2011) , 10.1007/S00262-011-0987-5