Expression and characterisation of recombinant human CD48 and isolation of a human anti‐CD48 monoclonal antibody by phage display
作者： Jiewei Wei , David Y Chin , Dallia Catzel , Natasha Pera , Stephen M Mahler
DOI: 10.1002/JCTB.1238
关键词:
摘要: Human CD48, a membrane-bound, glycosylphosphatidylinositol (GPI)-linked glycoprotein, is potential tumour target for the treatment of leukaemias and lymphomas. CD48 expressed on T-and B-cells, however < 5% CD34(+) progenitor cells express CD48. A truncated, 45kDa soluble form full length was in Chinese hamster ovary (CHO) cells, shown to consist broad range charge isoforms, with most abundant isoforms between pI 4.5 5.0. The truncated bind antibodies raised against native, GPI-linked by surface plasmon resonance analysis. synthetic, human, scFv immunoglobulin gene library screened recombinant phage display, an antibody fragment, (designated N2A) isolated after four rounds biopanning. N2A reassembled as human IgG1 monoclonal antibody, CHO binding IgG1-N2A confirmed resonance. Flow cytometry studies Raji showed specificity conserved, presents lead candidate white blood cell malignancies. (c) 2005 Society Chemical Industry.
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A. Nissim, H.R. Hoogenboom, I.M. Tomlinson, G. Flynn, C. Midgley, D. Lane, G. Winter, Antibody fragments from a 'single pot' phage display library as immunochemical reagents. The EMBO Journal. ,vol. 13, pp. 692- 698 ,(1994) , 10.1002/J.1460-2075.1994.TB06308.X
Miguel Angel de la Fuente, Pilar Pizcueta, Marga Nadal, Jaime Bosch, Pablo Engel, CD84 Leukocyte Antigen Is a New Member of the Ig Superfamily Blood. ,vol. 90, pp. 2398- 2405 ,(1997) , 10.1182/BLOOD.V90.6.2398
K. Johnsson, L. Ge, Phage Display of Combinatorial Peptide and Protein Libraries and Their Applications in Biology and Chemistry Current Topics in Microbiology and Immunology. ,vol. 243, pp. 87- 105 ,(1999) , 10.1007/978-3-642-60142-2_5
ME Reff, K Carner, KS Chambers, PC Chinn, JE Leonard, R Raab, RA Newman, N Hanna, DR Anderson, Depletion of B cells in vivo by a chimeric mouse human monoclonal antibody to CD20 Blood. ,vol. 83, pp. 435- 445 ,(1994) , 10.1182/BLOOD.V83.2.435.435
Noah Scheinfeld, Adalimumab (HUMIRA): a review. Journal of Drugs in Dermatology. ,vol. 2, pp. 375- 377 ,(2003)
C. Flores-Flores, A. Nissim, S. Shochat, H. Soreq, Development of human antibody fragments directed towards synaptic acetylcholinesterase using a semi-synthetic phage display library. Journal of Neural Transmission-supplement. pp. 165- 179 ,(2002) , 10.1007/978-3-7091-6139-5_16
Daming Shan, Jeffrey A. Ledbetter, Oliver W. Press, Apoptosis of malignant human B cells by ligation of CD20 with monoclonal antibodies Blood. ,vol. 91, pp. 1644- 1652 ,(1998) , 10.1182/BLOOD.V91.5.1644
David G. Maloney, Antonio J. Grillo-López, Christine A. White, David Bodkin, Russell J. Schilder, James A. Neidhart, Nalini Janakiraman, Kenneth A. Foon, Tina-Marie Liles, Brian K. Dallaire, Ken Wey, Ivor Royston, Thomas Davis, Ronald Levy, IDEC-C2B8 (Rituximab) Anti-CD20 Monoclonal Antibody Therapy in Patients With Relapsed Low-Grade Non-Hodgkin's Lymphoma Blood. ,vol. 90, pp. 2188- 2195 ,(1997) , 10.1182/BLOOD.V90.6.2188
Hennie R. Hoogenboom, Greg Winter, By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro. Journal of Molecular Biology. ,vol. 227, pp. 381- 388 ,(1992) , 10.1016/0022-2836(92)90894-P
D. E. Staunton, D. A. Thorley-Lawson, Molecular cloning of the lymphocyte activation marker Blast-1. The EMBO Journal. ,vol. 6, pp. 3695- 3701 ,(1987) , 10.1002/J.1460-2075.1987.TB02703.X