Flexibility and thermal dynamic stability increase of dsDNA induced by Ru(bpy) 2 dppz 2+ based on AFM and HRM technique
作者： Fuchao Jia , Pascal Hébraud , Kezhen Han , Jing Wang , Xingguo Liang
DOI: 10.1186/S13065-019-0584-9
关键词:
摘要: Ru(bpy)2dppz2+ has been widely used as a probe for exploring the structure of double-stranded DNA (dsDNA). The flexibility change helix is important in many its biological functions but not well understood. Here, dsDNA caused by intercalation with was investigated using atomic force microscopy. At first, interactions between ruthenium complex and were characterized binding site size (p = 2.87 bp) constant (Ka = 5.9 * 107 M−1) determined relative extension equation McGhee von Hippel. By measuring intercalator-induced elongation mean square end-to-end distance at different molar ratios to dsDNA, changes persistence length under concentrations worm-like chain model. We found that decreased increasing concentration, demonstrating obviously enhanced due intercalation. Especially, changed greatly from 54 34 nm on changing ratio 0 0.2. speculated resulted local deformation or bending duplex. In addition, thermal dynamic stability measured high resolution melting method which revealed increase
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