Identification of transfer RNA suppressors in Escherichia coli: IV. Amber suppressor su+6 a double mutant of a new species of leucine tRNA☆☆☆
作者： Masami Yoshimura , Hachiro Inokuchi , Haruo Ozeki
DOI: 10.1016/0022-2836(84)90041-X
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摘要: An Escherichia coli DNA fragment containing an Su+6 amber suppressor gene (supP) was cloned into a lambda gt Ch vector by the shotgun method, selecting transducing phage pSu+6. Through prophage integration followed induction occurring at region of pSu+6 in Su- E. coli, counterpart carrying wild-type allele (Su degrees 6) isolated (lambda pSu 6). The fingerprint tRNA encoded 6 identical to that unidentified tRNAE previously reported (Ikemura & Ozeki, 1977). cloverleaf structure this determined combining results analysis and sequencing gene. Judging from anticodon 5'-CAA-3', Su identified as new type leucine isoacceptor coli. Unlike other suppressors analyzed, differed two nucleotides tRNA; one (CAA CUA) junction D- anticodon-stem (A27 G27). sequence revealed single stretch is flanked putative sequences promoter terminator. Thus copy constitutes solitary transcription unit. Southern blotting showed only per haploid genome Since can mutate suppressor, may be accounted dispensable species among tRNAs. Two possible open reading frames are found immediately following
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