Responses of mature and aged sympathetic neurons to laminin and NGF : An in vitro study

摘要: Whilst the potent effects of NGF and laminin on developing neurons are well documented, relatively little is known about of, or altered availability responsiveness to, these substances growth adult neurons. We have therefore examined this question using explant cultures sympathetic from superior cervical ganglion (SCG) mature aged rats. Explants were grown substrata containing different doses laminin, either with without added in culture medium FCS. Individually, had small neurite outgrowth length, which tended to be reduced old In contrast, presence exogenous exerted a powerful effect nerve was substantially greater than sum individual factors. This synergy evident all experimental groups greatest explants at high where comparable that The dose-response curve indicated responsiveness. These results suggest variations and/or NGF, together patterns neuronal responsiveness, may contribute impaired plasticity age.