EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)-A New Drought Tolerant Mutant Wheat Line.

作者: Maré Vlok , Anna-Maria Botha , Christopher A. Cullis , Marlon-Schylor L. le Roux , Nicolas Francois V. Burger

DOI: 10.3390/IJMS22105314

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摘要: Drought response in wheat is considered a highly complex process, since it multigenic trait; nevertheless, breeding programs are continuously searching for new varieties with characteristics drought tolerance. In previous study, we demonstrated the effectiveness of mutant known as RYNO3936 that could survive 14 days without water. this reveal another BIG8-1 can endure severe water deficit stress (21 water) superior characteristics. Phenotypically, plants had broader leaves, including densely packed fibrous root architecture was not visible WT parent plants. During mild (day 7) stress, maintain its relative content, chlorophyll maximum quantum yield PSII (Fv/Fm) and stomatal conductance, no phenotypic symptoms such wilting or senescence despite decrease soil moisture content. It only during moderate 14) 21) decline those variables evident. Furthermore, also displayed unique preservation metabolic activity, which confirmed by assessing accumulation free amino acids increase antioxidative enzymes (peroxidases glutathione S-transferase). Proteome reshuffling observed, allowing slow degradation essential proteins RuBisCO stress. The LC-MS/MS data revealed high abundance involved energy photosynthesis under well-watered conditions, particularly Serpin-Z2A Z2B, SGT1 Calnexin-like protein. However, after 21 mutants expressed ABC transporter permeases xylanase inhibitor protein, transport protecting cells, respectively. This study characterizes drought-tolerant suited programs.

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