Dielectrophoretic Forces Can be Exploited to Increase the Efficiency of Animal Cell Perfusion Cultures

摘要: Dielectrophoresis is a well established and effective means for the manipulation of viable cells. Various applications have been found, ranging from electrofusion, to individual cell manipulation, differential separation mixtures. Its effectiveness, however, greatly depends upon utilization very low electrical conductivity media. High media, as in case with culture media result only negative dielectrophoresis (i.e., induction weaker repulsive forces) excessive medium heating. Recently, dielectrophoresis-based device (DEP-filter) has developed perfusion cultures that successfully overcomes these problems provides high degree while most nonviable cells are removed bioreactor by effluent stream. The latter results viabilities throughout period minimization lysed proteases bioreactor. However, an important question remains be answered whether we any adverse effects exposing cultured frequency dielectrophoretic fields extended periods time. A special chamber was constructed quantitate effect under several operational conditions. Cell growth, glucose, lactate monoclonal antibody production data suggest there no appreciable hence, operation over long time our DEP-filter should not on