Controlling DNA degradation from a distance: a new role for the Mu transposition enhancer

摘要: Summary Phage Mu is unique among transposable elements in employing a transposition enhancer. The enhancer DNA segment the site where transposase MuA binds and makes bridging interactions with two ends, interwrapping ends complex topology essential for assembling catalytically active transpososome. also at which regulatory proteins control divergent transcription of genes that determine phage lysis-lysogeny decision. Here we report third function – regulating degradation extraneous attached to both infecting Mu. This protected from nucleases by protein until integrates into host chromosome, after it rapidly degraded. We find leftward enhancer, expected disrupt its within transpososome, blocks this DNA. Disruption would lead loss or dislocation non-catalytic subunits positioned transpososome provide several lines support inference, conclude these are important activating flanking work reveals role development.