Novel Polymeric Hybrid Nanocarrier for Curcumin and Survivin shRNA Co-delivery Augments Tumor Penetration and Promotes Synergistic Tumor Suppression

摘要: A major barrier for co-delivery of gene medicine with small molecular chemotherapeutic drugs in solid tumors is the inadequate tumor penetration and transfection. In this study, a novel polymeric nanocarrier integrated properties penetration, nuclear targeting, pH-responsive features was designed, further used to achieve synergistic anti-tumor effect curcumin (CUR) survivin shRNA (pSUR). The hybrid constructed from FDA-approved polymer PLGA conjugated triblock W5R4K-PEG2K-PHIS (WPH). CUR pSUR were simultaneously encapsulated dual-drug-loaded nanoparticles (CUR/pSUR-NPs) by modified double-emulsion solvent evaporation (W/O/W) method. obtained exhibited better pharmaceutical uniform spherical morphology sustained release manners pSUR. Excellent including preferable cellular uptake, efficient endosomal escape, enhanced elevated transfection efficiency proven. Additionally, markedly efficacy CUR/shRNA-NPs achieved on SKOV-3 Hela cells. involved inhibition cell proliferation, induction apoptosis, activation caspase-3 pathways. This work sets up an innovative nanosystem suppress growth, contributing development comprehensive nanoparticulate strategy future clinical applications.