作者: Kathrin Hartelt , Rainer Oehme , Henning Frank , Stefan O. Brockmann , Dieter Hassler
DOI: 10.1016/S1433-1128(04)80013-5
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摘要: Abstract Tick-transmitted diseases like tick-borne encephalitis and Lyme borreliosis have been well known in Germany for decades. Ongoing research now gives an additional focus to a broad range of other bacteria parasites ticks Anaplasma phagocytophilum , former Ehrlichia sp., Rickettsia sp. Babesia Knowledge about the prevalence these infectious agents is important prerequisite risk assessment human diseases. Therefore nymphs adult Ixodes ricinus were collected examined (n = 5424 ticks), 1187), - 3113). For detection DNA from 16S rDNA gene was amplified by nested PCR hybridized with DIG-labeled oligonucleotide probe. The examination performed single PCR. A partial sequence citrate synthase amplified. As target 18S amplified, also All positive products sequenced control specificity. detected n 103 (1.9%) out 5,424 11 investigation areas. However, not all using Thus, result sequencing indicated that only 1.0% 54) belonged nearly half (0.9%) identified as Wolbachia 3 areas found 105 (8.9%) 1,187 (range 13.3% 5.6%). Sequencing showed helvetica exclusively. In 2.6% -positive ticks, double infection found. 31 (1.0%) 3,113 examined, which originated 4 different By sequencing, 28 (90.0%) divergens . Three — harboring microti phagocytophilum, demonstrates their possible role source Germany.