Molecular characterization of a copper transport protein in S. cerevisiae: An unexpected role for copper in iron transport

摘要: We report the identification and characterization of CTR1, a gene in yeast S. cerevisiae that encodes multispanning plasma membrane protein specifically required for high affinity copper transport into cell. The predicted contains methionine- serine-rich domain includes 11 examples sequence Met-X2-Met, motif noted proteins involved bacterial metabolism. CTR1 mutants deletion strains have profound deficiency ferrous iron uptake, thus revealing requirement mediating Genetic evidence suggests target this is FET3 (detailed companion study), to encode copper-containing acts as cytosolic ferro-oxidase. These findings provide an unexpected mechanistic link between uptake iron.