A subset of papillary thyroid carcinomas contain KRAS mutant subpopulations at levels above normal thyroid.

摘要: The molecular pathogenesis of papillary thyroid carcinoma (PTC) is largely attributed to chromosomal rearrangements and point mutations in genes within the MAPK pathway (i.e., BRAF RAS). Despite KRAS being 6th most frequently mutated gene for all cancers, reported frequency cancer only 2%. This may be due, part, use insensitive mutation detection methods such as DNA sequencing. Therefore, using sensitive quantitative ACB-PCR approach, we quantified codon 12 GGT  GAT GGT  GTT mutant fraction (MF) 20 normal tissues, 17 primary PTC, 2 metastatic 1 anaplastic carcinoma. We observed measurable levels GAT or GTT tissues. For PTCs, 29.4% 35.3% had MF above 95% upper confidence interval corresponding MFs thyroid. highest were associated with tumors follicular characteristics relatively high tumor necrosis. results indicate subpopulations are present a large number tumors, fact previously unrecognized. presence an aggressive phenotype, thus directing course clinical treatment. © 2012 Wiley Periodicals, Inc.