Molecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNA
作者： Harinder Singh , Jonathan H. LeBowitz , Albert S. Baldwin , Phillip A. Sharp
DOI: 10.1016/S0092-8674(88)80034-5
关键词:
摘要: Summary A novel strategy has been used to isolate a CDNA clone that encodes DNA binding domain whose recognition properties overlap those of the mammalian transcription factors H2TF1 and NF-κB. These two are distinguished by their cell type distributions relative affinities for related sequence elements in enhancers major histocompatibility complex (MHC) class 1 immunoglobulin κ chain genes. The human cDNA was detected screening λ phage expression library with site probe derived from MHC enhancer. encoded fusion protein binds specifically both gene enhancers. hybridizes single copy is expressed as 10 kb mRNA B non-B cells. this study may prove generally useful cloning analysis sequence-specific proteins.
sci-hub.se 参考文章(22)
David A. Johnson, James W. Gautsch, J.Richard Sportsman, John H. Elder, Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose Gene Analysis Techniques. ,vol. 1, pp. 3- 8 ,(1984) , 10.1016/0735-0651(84)90049-9
H. Towbin, T. Staehelin, J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 4350- 4354 ,(1979) , 10.1073/PNAS.76.9.4350
A S Baldwin, P A Sharp, Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene. Molecular and Cellular Biology. ,vol. 7, pp. 305- 313 ,(1987) , 10.1128/MCB.7.1.305
MM Bradford, None, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry. ,vol. 72, pp. 248- 254 ,(1976) , 10.1016/0003-2697(76)90527-3
Akinori Kimura, Alain Israël, Odile Le Bail, Philippe Kourilsky, Detailed analysis of the mouse H-2Kb promoter: enhancer-like sequences and their role in the regulation of class I gene expression Cell. ,vol. 44, pp. 261- 272 ,(1986) , 10.1016/0092-8674(86)90760-9
George Brawerman, Jozef Mendecki, Se Yong Lee, A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry. ,vol. 11, pp. 637- 641 ,(1972) , 10.1021/BI00754A027
Ranjan Sen, David Baltimore, Inducibility of κ immunoglobulin enhancer-binding protein NF-κB by a posttranslational mechanism Cell. ,vol. 47, pp. 921- 928 ,(1986) , 10.1016/0092-8674(86)90807-X
Dan R. Rawlins, Gregory Milman, S.Diane Hayward, Gary S. Hayward, Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region Cell. ,vol. 42, pp. 859- 868 ,(1985) , 10.1016/0092-8674(85)90282-X
Louis M. Staudt, Harinder Singh, Ranjan Sen, Thomas Wirth, Phillip A. Sharp, David Baltimore, A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes. Nature. ,vol. 323, pp. 640- 643 ,(1986) , 10.1038/323640A0
M Lenardo, J. Pierce, D Baltimore, Protein-binding sites in Ig gene enhancers determine transcriptional activity and inducibility Science. ,vol. 236, pp. 1573- 1577 ,(1987) , 10.1126/SCIENCE.3109035