Antiproliferative and Pro-apoptotic Activities of a Novel Resveratrol Prodrug Against Jurkat CD4+ T-Cells

摘要: BACKGROUND/AIM Resveratrol, a natural polyphenol, possesses many beneficial health properties but its therapeutic application is limited due to low water solubility and instability against oxidative processes. To improve the stability lipophilicity of compound, we synthesized resveratrol prodrug, termed FEHH4-1. In present study, compared antiproliferative pro-apoptotic effects with FEHH4-1 on Jurkat T-cells. MATERIALS AND METHODS Cell proliferation viability were monitored by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay, annexin-V/7-amino-actinomycin D staining western blot. induce interleukin-2 (IL2) expression, cells stimulated phorbol 12-myristate 13-acetate/phytohemagglutinin. IL2 production was quantified enzyme-linked immunosorbent assay. promoter activity studied T-cell line containing an luciferase reporter construct. RESULTS Both polyphenols inhibited proliferation, induced apoptotic cell death blocked synthesis in Most importantly, three-to four-times more potent than resveratrol. CONCLUSION had improved potential T-cells