The SNAG domain of Snail1 functions as a molecular hook for recruiting lysine‐specific demethylase 1

作者: Yiwei Lin , Yadi Wu , Junlin Li , Chenfang Dong , Xiaofeng Ye

DOI: 10.1038/EMBOJ.2010.63

关键词:

摘要: Epithelial–mesenchymal transition (EMT) is a transdifferentiation programme. The mechanism underlying the epigenetic regulation of EMT remains unclear. In this study, we identified that Snail1 interacted with histone lysine-specific demethylase 1 (LSD1). We demonstrated SNAG domain and amine oxidase LSD1 were required for their mutual interaction. Interestingly, sequence similar to H3 tail, interaction can be blocked by enzymatic inhibitors peptide. found formation Snail1–LSD1–CoREST ternary complex was critical stability function these proteins. co-expression molecules in cancer cell lines breast tumour specimens. Furthermore, showed recruiting its target gene promoters resulted suppression migration invasion. Our study suggests resembles H3-like structure functions as molecular hook repress expression metastasis.

参考文章(65)
Baiba Grube, Dujin Zhou, Thong K. Truong, Bin Yu, Toru Itoh, Shiuan Chen, Jin Zhao, Tomoharu Okubo, Down-regulation of promoter I.3 activity of the human aromatase gene in breast tissue by zinc-finger protein, Snail (SnaH) Cancer Research. ,vol. 61, pp. 1338- 1346 ,(2001)
Rhea T. Utley, Thomas A. Owen-Hughes, Li-Jung Juan, Jacques Côté, Christopher C. Adams, Jerry L. Workman, In vitro analysis of transcription factor binding to nucleosomes and nucleosome disruption/displacement Methods in Enzymology. ,vol. 274, pp. 276- 291 ,(1996) , 10.1016/S0076-6879(96)74024-7
Tracey A. Martin, Amit Goyal, Gareth Watkins, Wen G. Jiang, Expression of the Transcription Factors Snail, Slug, and Twist and Their Clinical Significance in Human Breast Cancer Annals of Surgical Oncology. ,vol. 12, pp. 488- 496 ,(2005) , 10.1245/ASO.2005.04.010
Jean Paul Thiery, Hervé Acloque, Ruby Y.J. Huang, M. Angela Nieto, Epithelial-Mesenchymal Transitions in Development and Disease Cell. ,vol. 139, pp. 871- 890 ,(2009) , 10.1016/J.CELL.2009.11.007
Jing Huang, Shelley L Berger, The emerging field of dynamic lysine methylation of non-histone proteins Current Opinion in Genetics & Development. ,vol. 18, pp. 152- 158 ,(2008) , 10.1016/J.GDE.2008.01.012
Masahiro Kajita, Karissa N. McClinic, Paul A. Wade, Aberrant expression of the transcription factors Snail and Slug alters the response to genotoxic stress Molecular and Cellular Biology. ,vol. 24, pp. 7559- 7566 ,(2004) , 10.1128/MCB.24.17.7559-7566.2004
Pamela Cowin, Tracey M Rowlands, Sarah J Hatsell, Cadherins and catenins in breast cancer Current Opinion in Cell Biology. ,vol. 17, pp. 499- 508 ,(2005) , 10.1016/J.CEB.2005.08.014
Maria J Blanco, Gema Moreno-Bueno, David Sarrio, Annamaria Locascio, Amparo Cano, José Palacios, M Angela Nieto, None, Correlation of Snail expression with histological grade and lymph node status in breast carcinomas. Oncogene. ,vol. 21, pp. 3241- 3246 ,(2002) , 10.1038/SJ.ONC.1205416
Anke Sparmann, Maarten van Lohuizen, Polycomb silencers control cell fate, development and cancer Nature Reviews Cancer. ,vol. 6, pp. 846- 856 ,(2006) , 10.1038/NRC1991
Alexander J. Ruthenburg, Haitao Li, Dinshaw J. Patel, C. David Allis, Multivalent engagement of chromatin modifications by linked binding modules Nature Reviews Molecular Cell Biology. ,vol. 8, pp. 983- 994 ,(2007) , 10.1038/NRM2298