GSM-900MHz at low dose temperature-dependently downregulates α-synuclein in cultured cerebral cells independently of chaperone-mediated-autophagy.

摘要: The expanding use of GSM devices has resulted in public concern. Chaperone-mediated autophagy (CMA) is a way for protein degradation the lysosomes and increases under stress conditions as cell defense response. α-synuclein, CMA substrate, component Parkinson disease. Since might constitute signal, we raised possibility that could alter process. Here, analyzed effects chronic exposure to low GSM-900MHz dose on apoptosis CMA. Cultured cerebral cortical cells were sham-exposed or exposed at specific absorption rate (SAR): 0.25W/kg 24 h using wire-patch cell. Apoptosis was by DAPI stain nuclei western blot cleaved caspase-3. expression proteins involved (HSC70, HSP40, HSP90 LAMP-2A) α-synuclein blot. also quantified situ analyzing localization active lysosomes. ∼0.5°C temperature rise. It did not induce but increased HSC70 26% slightly decreased (<10%). 24% independently CMA, since altered. Comparable observed incubated 37.5°C, condition mimics GSM-generated GSM-induced changes HSC70, are most likely linked We observe any immediate effect viability. However, delayed long term consequences (protective deleterious) these fate should be examined.