Insulin releasing effect of some pure compounds from Moringa oleifera on mice islets

摘要: The anti-diabetic activity of extracts, fractions and compounds Moringa oleifera have been reported; however, several constituents from this well known medicinal plant are not yet screened for bio-perspecting role diabetes. Current studies demonstrated the properties five chemical viz, 4-hydroxyphenylacetonitrite (1), fluoropyrazine (3), methyl-4-hydroxybenzoate (4), vanillin (5), 4-α-L-rhamnopyranosyloxybenzyl isothiocyanate (6) along with one related compound 3,4-dihydroxy benzonitrile (2) first time in vitro vivo. Furthermore, mechanism action was predicted by utilizing molecular docking protein kinase A (PKA) exchange activated cAMP (Epac2A). structure elucidated UV, IR, MS, 1H NMR. 1, 3–5 induced significant insulin secretion at stimulatory (16.7 mM) glucose, but basal (3 glucose concentration, 3 seems to be most active. Compounds showed dose-dependent secretory optimum response 200 μM. In silico revealed that has a noticeable electrostatic hydrophobic interaction (PKA). also there reduction 1–3 mediated presence PKA inhibitor suggesting is possible signaling pathway on secretion. Upon oral administration diabetic rats, 1 significantly reduced blood level rats dose- time-dependent manner. tolerance test enhanced plasma improved beta-cell function. cytotoxicity assay, did show any toxic effect upto releasing characteristic different M. conceivably correlate lowering vivo triggering glucose-induced pancreatic islets possibly PKA-mediated pathway.