Toxicological hazard induced by sucralose to environmentally relevant concentrations in common carp (Cyprinus carpio)
作者： Karinne Saucedo-Vence , Armando Elizalde-Velázquez , Octavio Dublán-García , Marcela Galar-Martínez , Hariz Islas-Flores
DOI: 10.1016/J.SCITOTENV.2016.09.230
关键词:
摘要: Sucralose (SUC) is an artificial sweetener that now widely used in North American and Europe; it has been detected a wide variety of aquatic environments. It considered safe for human consumption but its effects the ecosystem have not yet studied depth, since limited ecotoxicological data are available peer-reviewed literature. This study aimed to evaluate potential SUC-induced toxicological hazard blood, brain, gill, liver muscle Cyprinus carpio using oxidative stress biomarkers. Carps were exposed two different environmentally relevant concentrations (0.05 155μgL-1) exposure times (12, 24, 48, 72 96h). The following biomarkers evaluated: lipid peroxidation (LPX), hydroperoxide content (HPC) protein carbonyl (PCC), as well activity antioxidant enzymes, superoxide dismutase (SOD) catalase (CAT). SUC was determined by high pressure liquid chromatography tandem mass spectrometry techniques (HPLC)-MS/MS. Results show statically significant increase LPX, HPC, PCC (P<0.05) especially brain muscle, changes enzymes gill muscle. Furthermore, employed this useful assessment environmental impact agent on species.
harvard.edu
uaemex.mx参考文章(88)
Nely SanJuan-Reyes, Leobardo Manuel Gómez-Oliván, Marcela Galar-Martínez, Sandra García-Medina, Hariz Islas-Flores, Edgar David González-González, Jesús Daniel Cardoso-Vera, Juan Manuel Jiménez-Vargas, NSAID-manufacturing plant effluent induces geno- and cytotoxicity in common carp (Cyprinus carpio) Science of The Total Environment. ,vol. 530, pp. 1- 10 ,(2015) , 10.1016/J.SCITOTENV.2015.05.088
Gonzalez Mantilla, Jaime Fernando, Hepatic Phase I and II Biotransformation Kinetics in Fishes: A Comparative Study ,(2006)
H Grice✠, Sucralose--an overview of the toxicity data. Food and Chemical Toxicology. ,vol. 38, pp. 1- 6 ,(2000) , 10.1016/S0278-6915(00)00023-5
Rodney L. Levine, Joy A. Williams, Earl P. Stadtman, Emily Shacter, [37] Carbonyl assays for determination of oxidatively modified proteins Methods in Enzymology. ,vol. 233, pp. 346- 357 ,(1994) , 10.1016/S0076-6879(94)33040-9
R. Radi, J.F. Turrens, L.Y. Chang, K.M. Bush, J.D. Crapo, B.A. Freeman, Detection of catalase in rat heart mitochondria. Journal of Biological Chemistry. ,vol. 266, pp. 22028- 22034 ,(1991) , 10.1016/S0021-9258(18)54740-2
John A. Buege, Steven D. Aust, Microsomal lipid peroxidation. Methods in Enzymology. ,vol. 52, pp. 302- 310 ,(1978) , 10.1016/S0076-6879(78)52032-6
S.D. Yan, A.M. Schmidt, G.M. Anderson, J. Zhang, J. Brett, Y.S. Zou, D. Pinsky, D. Stern, Enhanced cellular oxidant stress by the interaction of advanced glycation end products with their receptors/binding proteins. Journal of Biological Chemistry. ,vol. 269, pp. 9889- 9897 ,(1994) , 10.1016/S0021-9258(17)36966-1
Oliver J. Mace, Julie Affleck, Nick Patel, George L. Kellett, Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2 The Journal of Physiology. ,vol. 582, pp. 379- 392 ,(2007) , 10.1113/JPHYSIOL.2007.130906
Hara P. Misra, Irwin Fridovich, The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase Journal of Biological Chemistry. ,vol. 247, pp. 3170- 3175 ,(1972) , 10.1016/S0021-9258(19)45228-9
Kenneth S. Lown, Steven A. Wrighton, Paul B. Watkins, Kenneth E. Thummel, Robert J. Fontana, David G. Bailey, Stephen D. Hall, Stephen D. Hall, Leslie Z. Benet, Mary F. Paine, Robert R. Mayo, D. Kim Turgeon, Molecular and physical mechanisms of first-pass extraction. Drug Metabolism and Disposition. ,vol. 27, pp. 161- 166 ,(1999)