In-house method validation : Application in arsenic analysis

摘要: Abstract A detailed procedure for in-house method validation was proposed and applied to the of a determination arsenic in bovine liver. Linearity matrix effects were assessed by solvent matrix-matched curves. Data fitted ordinary least squares (OLSM). Violations assumptions underlying OLSM residuals (normality, independence homoscedasticity) evaluated. F-tests undertaken check adjustment model evaluation regression lack fit significances. The slopes interceptions compared significant differences t-test. demonstrated not observed between 0.5 8 ng mL−1. Selectivity, trueness, precision experimental limits established assays with blanks spiked samples. Recovery (means 88.9–99.9%) calculated analysis variance carried out estimate standard deviation under repeatability (3.7–7.2%) within-reproducibility (3.7–7.3%) conditions. spiking experiments indicated no trueness. Experimental detection quantification 15 30 μg kg−1, respectively. found be suitable respect efficiency parameters, ease application cost-benefits.