Universal vaccine against influenza virus: linking TLR signaling to anti-viral protection.
作者： Nicole Schmitz , Roger R. Beerli , Monika Bauer , Andrea Jegerlehner , Klaus Dietmeier
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摘要: A vaccine protecting against all influenza strains is a long-sought goal, particularly for emerging pandemics. As previously shown, vaccines based on the highly conserved extracellular domain of M2 (M2e) may protect strains. Here, we demonstrate that M2e-specific monoclonal antibodies (mAbs) mice from lethal infection. To be protective, had to able bind Fc receptors and fix complement. Furthermore, mAbs IgG2c isotype were protective in mice, while identical specificity, but IgG1 isotype, failed prevent disease. These findings readily translated into design. targeting absence toll-like receptor (TLR) 7 ligand primarily induced IgG1, whilst same linked TLR7 yielded high levels antibodies. Although both protected challenge, treated with containing showed significantly lower morbidity. In accordance these findings, vaccination TLR7(-/-) did not result protection challenge. Hence, innate immune system required direct switching toward more IgG2a/c
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