Defective Interfering Passages of Sindbis Virus: Chemical Composition, Biological Activity, and Mode of Interference

摘要: Defective interfering (DI) particles of Sindbis virus, appearing between the eighth and fourteenth passages, cosediment with have same buoyant density as standard virus. Virion RNA from such late passages is heterogeneous by polyacrylamide gel electrophoresis, whereas early passage homogeneous. No differences were found in virion proteins passages. Cells co-infected virus synthesize much intracellular viral-specific protein made after infection alone, although production inhibited 90% or more. Such cells two new species molecular weights 2.2 x 10(6) 0.86 10(6). Nucleocapsid assembly blocked these cells, amount capsid reduced 50%. The presence a was detected electrophoresis.