Immunoenzymatic visualization of tetrodotoxin (TTX) in Cephalothrix species (Nemertea: Anopla: Palaeonemertea: Cephalotrichidae) and Planocera reticulata (Platyhelminthes: Turbellaria: Polycladida: Planoceridae).

作者: Mohosena Begum Tanu , Yahia Mahmud , Osamu Arakawa , Tomohiro Takatani , Hiroshi Kajihara

DOI: 10.1016/J.TOXICON.2004.06.014

关键词:

摘要: Tetrodotoxin (TTX) was localized as brown color in different tissues of an undescribed species the nemertean genus Cephalothrix (phylum Nemertea) and a turbellarian Planocera reticulata Platyhelminthes) on light microscopy by means monoclonal anti-TTX antibody. In sp., TTX recognized vesicles apically arranged bacillary cells epidermis, basal lamina, granular proboscis epithelium, rhynchocoel portion intestinal wall near blood vessels rhynchocoel. The excretory system ovum also showed positive reaction antigen-antibody. On other hand, hermaphrodite flatworm P. exhibited antigen-antibody complex only their ovum. To our knowledge, this is first experimental effort micro-distribution invertebrates.

参考文章(27)
Deng-Fwu Hwang, Chao-An Cheng, Hsing-Chen Chen, Sen-Shyong Jeng, Tamao Noguchi, Kouichi Ohwada, Kanehisa Hashimoto, Microflora and Tetrodotoxin-producing Bacteria in the Lined Moon Shell Natica lineata. Fisheries Science. ,vol. 60, pp. 567- 571 ,(1994) , 10.2331/FISHSCI.60.567
Erik V. Thuesen, Kazuhiro Kogure, Kanehisa Hashimoto, Takahisa Nemoto, Poison arrowworms: a tetrodotoxin venom in the marine phylum Chaetognatha Journal of Experimental Marine Biology and Ecology. ,vol. 116, pp. 249- 256 ,(1988) , 10.1016/0022-0981(88)90030-5
William R. Kem, Bernard C. Abbott, Robert M. Coates, Isolation and structure of a hoplonemertine toxin. Toxicon. ,vol. 9, pp. 15- 22 ,(1971) , 10.1016/0041-0101(71)90039-0
Yahia Mahmud, Kentaro Okada, Tomohiro Takatani, Kentaro Kawatsu, Yonekazu Hamano, Osamu Arakawa, Tamao Noguchi, Intra-tissue distribution of tetrodotoxin in two marine puffers Takifugu vermicularis and Chelonodon patoca. Toxicon. ,vol. 41, pp. 13- 18 ,(2003) , 10.1016/S0041-0101(02)00169-1
Keisuke Miyazawa, Masaru Higashiyama, Keiji Ito, Tamao Noguchi, Osamu Arakawa, Yasuo Shida, Kanehisa Hashimoto, Tetrodotoxin in two species of ribbon worm (Nemertini), Lineus fuscoviridis and Tubulanus punctatus Toxicon. ,vol. 26, pp. 867- 874 ,(1988) , 10.1016/0041-0101(88)90327-3
Kazumi Tsuruda, Osamu Arakawa, Kentaro Kawatsu, Yonekazu Hamano, Tomohiro Takatani, Tamao Noguchi, Secretory glands of tetrodotoxin in the skin of the Japanese newt Cynops pyrrhogaster. Toxicon. ,vol. 40, pp. 131- 136 ,(2002) , 10.1016/S0041-0101(01)00198-2
JON L. NORENBURG, Structure of the nemertine integument with consideration of its ecological and phylogenetic significance Integrative and Comparative Biology. ,vol. 25, pp. 37- 51 ,(1985) , 10.1093/ICB/25.1.37
Manabu Asakawa, Tadayoshi Toyoshima, Yasuo Shida, Tamao Noguchi, Keisuke Miyazawa, Paralytic toxins in a ribbon worm Cephalothrix species (Nemertean) adherent to cultured oysters in Hiroshima Bay, Hiroshima Prefecture, Japan. Toxicon. ,vol. 38, pp. 763- 773 ,(2000) , 10.1016/S0041-0101(99)00172-5
Yahia Mahmud, Osamu Arakawa, Akitoyo Ichinose, Mohosena Begum Tanu, Tomohiro Takatani, Kazumi Tsuruda, Kentaro Kawatsu, Yonekazu Hamano, Tamao Noguchi, Intracellular visualization of tetrodotoxin (TTX) in the skin of a puffer Tetraodon nigroviridis by immunoenzymatic technique Toxicon. ,vol. 41, pp. 605- 611 ,(2003) , 10.1016/S0041-0101(03)00003-5