Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens.

摘要: We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through production alloreactive-CD8 T and -memory CD8 cells, based on their leukocyte antigen (HLA) expression. In surface analysis, ADSCs do not express co-stimulatory molecules, but expresses HLA-ABC, which is further increased by exposure to pro-inflammatory cytokines as well IFN-γ alone. For cultured in xenofree medium (XF-ADSCs) were incubated with recipient immune for allogeneic-antigen stimulation. As a result, XF-ADSCs induced IL-17A release cell direct pathway, although they have immunomodulatory activity. analysis alloreactive memory also significantly CFSE-low-CD8 TEM -CD8 TCM cells. However, HLA-blocking antibodies inhibited CFSE-low memory-CD8 indicating that HLAs are main antigens responsible development ADSCs' immunogenicity. These results suggested HLA expressed MSCs should be solved order address concerns related problem.