Effect of ethylene-diamine-tetra-acetic acid (dipotassium salt) and heparin on the estimation of packed cell volume

作者: C. A. Pennock , K. W. Jones

DOI: 10.1136/JCP.19.2.196

关键词:

摘要: The effect of varying concentrations ethylene-diamine-tetra-acetic acid (E.D.T.A.) (dipotassium salt) and heparin on the estimation packed cell volume has been studied using a microhaematocrit method. Varying E.D.T.A. can produce serious errors in (P.C.V.) reliable results are only obtained within range 1 to 2 mg./ml. Heparin is more suitable anticoagulant for this investigation as concentration little effect. Storage with either at 24 hours room temperature influence results.

参考文章(10)
H. CHAPLIN, P. L. MOLLISON, Correction for plasma trapped in the red cell column of the hematocrit. Blood. ,vol. 7, pp. 1227- 1238 ,(1952) , 10.1182/BLOOD.V7.12.1227.1227
L. Garby, J.-C. Vuille, The Amount of Trapped Plasma in a High Speed Micro-Capillary Hematocrit Centrifuge Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 13, pp. 642- 645 ,(1961) , 10.3109/00365516109137338
D. Leeson, E. B. Reeve, The plasma in the packed cell column of the haematocrit The Journal of Physiology. ,vol. 115, pp. 129- 142 ,(1951) , 10.1113/JPHYSIOL.1951.SP004657
R. Biggs, R. L. Macmillan, The Errors of Some Haematological Methods as They Are Used in a Routine Laboratory Journal of Clinical Pathology. ,vol. 1, pp. 269- 287 ,(1948) , 10.1136/JCP.1.5.269
Eric Ponder, George Saslow, The measurement of red cell volume The Journal of Physiology. ,vol. 70, pp. 18- 37 ,(1930) , 10.1113/JPHYSIOL.1930.SP002673
R. F. Jones, Determination of packed cell volume by centrifugation. Journal of Clinical Pathology. ,vol. 14, pp. 198- 199 ,(1961) , 10.1136/JCP.14.2.198
Max M. Strumia, Albert B. Sample, Eleanor D. Hart, An improved micro hematocrit method. American Journal of Clinical Pathology. ,vol. 24, pp. 1016- 1024 ,(1954) , 10.1093/AJCP/24.9.1016
I. Kaldor, A MICROTECHNIQUE FOR MEASURING THE HÆMATOCRIT VALUE Medical Journal of Australia. ,vol. 1, pp. 476- 478 ,(1953) , 10.5694/J.1326-5377.1953.TB79054.X
G. Gordon Hadley, Norman L. Larson, Use of sequestrene as an anticoagulant. American Journal of Clinical Pathology. ,vol. 23, pp. 613- 618 ,(1953) , 10.1093/AJCP/23.6_TS.613
James A. Lampasso, ERROR IN HEMATOCRIT VALUE PRODUCED BY EXCESSIVE ETHYLENEDIAMINETETRAACETATE. American Journal of Clinical Pathology. ,vol. 44, pp. 109- 110 ,(1965) , 10.1093/AJCP/44.1-TS.109