In vivo Hox binding specificity revealed by systematic changes to a single cis regulatory module

摘要: Hox proteins belong to a family of transcription factors with similar DNA binding specificities that control animal differentiation along the antero-posterior body axis. are expressed in partially overlapping regions where each one is responsible for formation particular organs and structures through regulation specific direct downstream targets. Thus, explaining how protein can selectively its targets from those another fundamental understand development. Here we analyse cis regulatory module directly regulated by seven different Drosophila uncover class differentially expression. We find or depends on combination three modes: Hox-cofactor dependent DNA-binding specificity; Hox-monomer sites; interaction positive negative Hox-collaborator proteins. Additionally, be achieved Amphioxus orthologs, suggesting these mechanisms conserved insects chordates.