A Stachybotrys chartarum isolate from soybean.

摘要: As part of our effort to investigate fungi associated with soybean roots, Stachybotrys chartarum was isolated from root lesions. Since this fungus has not been reported cause a disease soybean, the objectives were identify and characterize using biological, chemical, molecular approaches. Fungal morphology examined light environmental scanning electron microscopy. Phialides bearing conidia arose determinate, macronematous, dark olivaceous conidiophores. The phialides obovate or ellipsoidal in whorls. Conidia unicellular, round ellipsoidal, 5–13 × 4–7 μm, initially hyaline smooth walls then brown black rough-walled when mature. Radial growth on cornmeal, oatmeal potato dextrose agar 38, 47, 33 mm diam., respectively, after 10 days at 25 °C. Pathogenicity performed sorghum grain colonized by S. placed below sown seeds soil : sand (1 1) steam-pasteurized mix. Three weeks inoculation, lesions ranged 7 long. reisolated reidentified as chartarum. Biochemical analysis indicated that isolate produced satratoxins G H along roridin L-2, well spircyclic lactones lactams rice culture. PCR chartarum-specific primer StacR3 IT51 amplified 198-bp DNA fragment total genomic DNA. sequence ITS region 100% identical strain ATCC 9182, one nucleotide mismatch UAMH 7900, differed all published sequences 12 other species 2 Memnoniella GenBank genetic divergence ranging 5.26 9.98%. This evidence further supports identification