Interaction between genomes of infectious bronchitis and Newcastle disease viruses studied by reverse transcription-polymerase chain reaction.

摘要: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for the S1 gene of IBV and fusion protein cleavage site NDV was used detection Infectious bronchitis virus (IBV, family Coronaviridae) Newcastle disease (NDV) genomes. The sensitivity RT-PCR 10(3.7) 10(3.0) EID50, respectively. Although a multiplex could detect differentiate genomes present in same sample, there slight inhibition PCR if high amount genome sample. To overcome this problem separate each to assess interaction between vaccine either inoculated singly or together into chickens. In group vaccinated (ND) alone, viral detected on days 2, 4 7 post vaccination (p.v.), while chickens given infectious (IB) only day p.v. both viruses 10(3)-fold reduction cDNA dilution factor This demonstrated clearly that when these vaccines are administered is transient replication viruses, probably due their competition target epithelial cells respiratory tract.