[25] Primer extension analysis of RNA

作者: William R. Boorstein , Elizabeth A. Craig

DOI: 10.1016/0076-6879(89)80111-9

关键词:

摘要: … Buffers and solutions used for hybridization and primer extension reactions should be RNase free. RNases can be inactivated by the addition of diethyl pyrocarbonate (DEPC) to a final …

参考文章(11)
P J Good, R C Welch, W S Ryu, J E Mertz, The late spliced 19S and 16S RNAs of simian virus 40 can be synthesized from a common pool of transcripts. Journal of Virology. ,vol. 62, pp. 563- 571 ,(1988) , 10.1128/JVI.62.2.563-571.1988
Max L. Birnstiel, Bruce H. Sells, Ian F. Purdom, Kinetic complexity of RNA molecules. Journal of Molecular Biology. ,vol. 63, pp. 21- 39 ,(1972) , 10.1016/0022-2836(72)90519-0
A.A. Szalay, K. Grohmann, R.L. Sinsheimer, Separation of the complementary strands of DNA fragments on polyacrylamide gels. Nucleic Acids Research. ,vol. 4, pp. 1569- 1578 ,(1977) , 10.1093/NAR/4.5.1569
D. A. MILLER, P. W. ALLDERDICE, O. J. MILLER, W. R. BREG, Quinacrine fluorescence patterns of human D group chromosomes. Nature. ,vol. 232, pp. 24- 27 ,(1971) , 10.1038/232024A0
Johan R. Lillehaug, Ruth K. Kleppe, Kjell Kleppe, Phosphorylation of double-stranded DNAs by T4 polynucleotide kinase Biochemistry. ,vol. 15, pp. 1858- 1865 ,(1976) , 10.1021/BI00654A011
James Casey, Norman Davidson, Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide Nucleic Acids Research. ,vol. 4, pp. 1539- 1552 ,(1977) , 10.1093/NAR/4.5.1539
Otto Hagenbüchle, Melvin Santer, Joan Argetsinger Steitz, Rusty J. Mans, Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cells Cell. ,vol. 13, pp. 551- 563 ,(1978) , 10.1016/0092-8674(78)90328-8