Determination and Quantification of Molecular Interactions in Protein Films: A Review

摘要: Protein based films are nowadays also prepared with the aim of replacing expensive, crude oil-based polymers as environmentally friendly and renewable alternatives. The protein structure determines ability chains to form intra- intermolecular bonds, whereas degree cross-linking depends on amino acid composition molecular weight protein, besides conditions used in film preparation processing. functionality varies significantly depending type affects resulting quality properties. This paper reviews methods examination interactions discusses how these can be quantified. qualitative determination distinguished by structural analysis solutions (electrophoretic analysis, size exclusion chromatography) solid (spectroscopy techniques, X-ray scattering methods). To quantify involved, two were found most suitable: swelling solubility. importance non-covalent covalent investigated using different solvents. research was focused whey soy wheat gluten included further examples proteins.