Targeted tumor drug delivery and magnetic hyperthermia for cancer treatment by chemotherapeutic-conjugated magnetic nanoparticles

摘要: One of the promising strategies for improvement cancer treatment is based on magnetic drug delivery systems, thus avoiding side effects standard chemotherapies. Superparamagnetic iron oxide (SPIONs) nanoparticles have ideal properties to become a targeted contrast probes, named theranostics. The aim this work was investigate in vitro effect loading doxorubicin (DOX) negatively charged polycarboxylic iron-oxide breast carcinoma cell lines. For proper analysis and understanding behavior after administration DOX-SPIONs compared with free DOX, complex set tests, including production MTT assay, cycle determination, cellular uptake, were utilized. In summary, we developed nanoparticle-based system that sequentially delivers cytotoxic cells (MCF-7 MDA-MB-231). drug-coated nanoparticles, DOX-NPs, assembled stepwise, adsorbed bare first, by electrostatic reaction allowed complexation doxorubicin. DOX-NPs stable solution at 37 °C physiological pH (7.4). Rapid release followed gradual triggered aqueous low (5.4) heating. As result minimal internalization, particles not significantly toxic noncancerous (MCF-10A). contrast, they internalized much greater extent MCF-7 MDA-MB-231 due synergistic action two drugs hyperthermia. able inhibit growth proliferation vitro, indicating has potential act as an antimetastatic chemothermotherapeutic agent.One with...