Downregulation by tumor necrosis factor-α of monocyte CCR2 expression and monocyte chemotactic protein-1-induced transendothelial migration is antagonized by oxidized low-density lipoprotein
作者： Christian Weber , Georg Draude , Kim S.C. Weber , Joachim Wübert , Reinhard L. Lorenz
DOI: 10.1016/S0021-9150(99)00021-0
关键词:
摘要: The subintimal infiltration with monocytes is crucially involved in the development of complex atherosclerotic plaques. Monocyte chemotactic protein-1 (MCP-1) and its receptor CCR2 are important for monocyte extravasation formation lesions. However, mechanisms persistence plaques remain to be elucidated. Flow cytometric analysis revealed that monocytoid Mono Mac 6 cells had transmigrated endothelium towards a MCP-1 gradient expressed higher levels than non-migratory fraction, while input were intermediate, suggesting high essential transendothelial chemotaxis. Pretreatment or isolated human blood inflammatory cytokine tumor necrosis factor (TNF)-alpha dose- time-dependently reduced MCP-1-induced chemotaxis, which was inhibited by antagonist 9-76 analog. This paralleled decrease surface protein mRNA expression. as assessed flow cytometry reverse transcription-polymerase chain reaction, inferring inhibition transmigration due downregulation insufficient In contrast, treatment oxidized low-density (oxLDL) containing lipids, such cholesteryl linoleate 13-hydroxide. but not LDL, increased Notably, oxLDL counteracted TNF-alpha-mediated CCR2-dependent Macrophage-colony-stimulating hardly affected expression function, differentiation responsible effects on CCR2. conclusion, TNF-alpha impairs migration downregulating appears critical migration. Exposure antagonized TNF-alpha, may thus contribute retention perpetuation chronic reaction unstable
elsevier.com
sci-hub.se 参考文章(40)
Daniel Steinberg, Oxidative Modification of LDL and Atherogenesis Circulation. ,vol. 95, pp. 141- 147 ,(1998) , 10.1007/978-94-011-5022-4_16
E. R. Stanley, P. Roth, The biology of CSF-1 and its receptor. Current Topics in Microbiology and Immunology. ,vol. 181, pp. 141- 167 ,(1992) , 10.1007/978-3-642-77377-8_5
Landin Boring, Jennifa Gosling, Michael Cleary, Israel F. Charo, Decreased lesion formation in CCR2 −/− mice reveals a role for chemokines in the initiation of atherosclerosis Nature. ,vol. 394, pp. 894- 897 ,(1998) , 10.1038/29788
T A Springer, J M Casasnovas, C Weber, C F Lu, Role of alpha L beta 2 integrin avidity in transendothelial chemotaxis of mononuclear cells. Journal of Immunology. ,vol. 159, pp. 3968- 3975 ,(1997)
Marco Baggiolini, Chemokines and leukocyte traffic Nature. ,vol. 392, pp. 565- 568 ,(1998) , 10.1038/33340
Marco Baggiolini, Beatrice Dewald, Bernhard Moser, Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines. Advances in Immunology. ,vol. 55, pp. 97- 179 ,(1994) , 10.1016/S0065-2776(08)60509-X
Dominique Stengel, Micheline Antonucci, Muriel Arborati, Delphine Hourton, Sabine Griglio, M. John Chapman, Ewa Ninio, Expression of the PAF receptor in human monocyte-derived macrophages is downregulated by oxidized LDL: relevance to the inflammatory phase of atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology. ,vol. 17, pp. 954- 962 ,(1997) , 10.1161/01.ATV.17.5.954
L. Kritharides, W. Jessup, J. Gifford, R.T. Dean, A method for defining the stages of low-density lipoprotein oxidation by the separation of cholesterol- and cholesteryl ester-oxidation products using HPLC Analytical Biochemistry. ,vol. 213, pp. 79- 89 ,(1993) , 10.1006/ABIO.1993.1389
C. Weber, W. Erl, P.C. Weber, Enhancement of Monocyte Adhesion to Endothelial Cells by Oxidatively Modified Low-Density Lipoprotein Is Mediated by Activation of Cd11B Biochemical and Biophysical Research Communications. ,vol. 206, pp. 621- 628 ,(1995) , 10.1006/BBRC.1995.1088
B G Ohlsson, M C Englund, A L Karlsson, E Knutsen, C Erixon, H Skribeck, Y Liu, G Bondjers, O Wiklund, Oxidized low density lipoprotein inhibits lipopolysaccharide-induced binding of nuclear factor-kappaB to DNA and the subsequent expression of tumor necrosis factor-alpha and interleukin-1beta in macrophages. Journal of Clinical Investigation. ,vol. 98, pp. 78- 89 ,(1996) , 10.1172/JCI118780