Age-, gender-, and species-dependent mutagenicity in T cells of mice and rats exposed by inhalation to 1,3-butadiene

作者: Quanxin Meng , Dale M. Walker , Jake D. McDonald , Rogene F. Henderson , Meghan M. Carter

DOI: 10.1016/J.CBI.2006.07.005

关键词:

摘要: Experiments were performed: (i) to investigate potential age- and gender-dependent differences in mutagenic responses T cells following exposures of B6C3F1 mice F344 rats by inhalation for 2 weeks 0 or 1250 ppm butadiene (BD), (ii) determine if 62.5 BD produce a effect female rats. To evaluate the age on response, mutant manifestation curves splenic exposed at 8-9 defined measuring Hprt frequencies (MFs) multiple time points after exposure using cell cloning assay comparing resulting potency estimate (calculated as difference areas under treated versus control animals) that reported same fashion beginning 4-5 age. The shapes spleens different [e.g., maximum BD-induced MFs older (8.0+/-1.0 [S.D.]x10(-6)) younger (17.8+/-6.1 x 10(-6)) observed 8 5 post-exposure, respectively], but burden was both groups. assess gender male rodents measured when are expected occur post-exposure. data demonstrated pattern susceptibility from high-level is mice>male mice>female rats>male Exposures caused minor significant response compared with controls (n=16/group; P=0.03). These results help explain part differing outcomes/interpretations earlier mutation studies BD-exposed rodents.

参考文章(35)
Thomas R. Skopek, Teresa R. Craft, Johan G. deBoer, Vernon E. Walker, Julie L. Andrews, Barry W. Glickman, Nancy J. Gorelick, Frequency and Spectrum of Ethylnitrosourea-induced Mutation at the hprt and lacI Loci in Splenic Lymphocytes of Exposed lacI Transgenic Mice Cancer Research. ,vol. 56, pp. 4654- 4661 ,(1996)
Tao Chen, Robert H Heflich, Dale M Walker, Michael J Bauer, Andrew A Reilly, Vernon E Walker, Quanxin Meng, Rogene F Henderson, Mutagenicity of 1,3-butadiene at the Hprt locus of T-lymphocytes following inhalation exposures of female mice and rats Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. ,vol. 429, pp. 107- 125 ,(1999) , 10.1016/S0027-5107(99)00104-9
Irene M. Jones, Karolyn Burkhart-Schultz, Tawni L. Crippen, Cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo. Somatic Cell and Molecular Genetics. ,vol. 13, pp. 325- 333 ,(1987) , 10.1007/BF01534926
J. Huff, R. Melnick, H. Solleveld, J. Haseman, M Powers, R. Miller, Multiple organ carcinogenicity of 1,3-butadiene in B6C3F1 mice after 60 weeks of inhalation exposure Science. ,vol. 227, pp. 548- 549 ,(1985) , 10.1126/SCIENCE.3966163
Quanxin Meng, Rogene F Henderson, Lingyan Long, Lee Blair, Dale M Walker, Patricia B Upton, James A Swenberg, Vernon E Walker, Mutagenicity at the Hprt locus in T cells of female mice following inhalation exposures to low levels of 1,3-butadiene Chemico-Biological Interactions. ,vol. 135-136, pp. 343- 361 ,(2001) , 10.1016/S0009-2797(01)00222-8
Anane Aidoo, Lascelles E. Lyn-Cook, Roberta A. Mittelstaedt, Robert H. Heflich, Daniel A. Casciano, J. Cole, Induction of 6-thioguanine-resistant lymphocytes in Fischer 344 rats following in vivo exposure to N-ethyl-N-nitrosourea and cyclophosphamide. Environmental and Molecular Mutagenesis. ,vol. 17, pp. 141- 151 ,(1991) , 10.1002/EM.2850170302
Quanxin Meng, Thomas R. Skopek, Dale M. Walker, Shellene Hurley-Leslie, Tao Chen, David M. Zimmer, Vernon E. Walker, Culture and propagation of Hprt mutant T-lymphocytes isolated from mouse spleen. Environmental and Molecular Mutagenesis. ,vol. 32, pp. 236- 243 ,(1998) , 10.1002/(SICI)1098-2280(1998)32:3<236::AID-EM6>3.0.CO;2-Y