作者: Quanxin Meng , Dale M. Walker , Jake D. McDonald , Rogene F. Henderson , Meghan M. Carter
DOI: 10.1016/J.CBI.2006.07.005
关键词:
摘要: Experiments were performed: (i) to investigate potential age- and gender-dependent differences in mutagenic responses T cells following exposures of B6C3F1 mice F344 rats by inhalation for 2 weeks 0 or 1250 ppm butadiene (BD), (ii) determine if 62.5 BD produce a effect female rats. To evaluate the age on response, mutant manifestation curves splenic exposed at 8-9 defined measuring Hprt frequencies (MFs) multiple time points after exposure using cell cloning assay comparing resulting potency estimate (calculated as difference areas under treated versus control animals) that reported same fashion beginning 4-5 age. The shapes spleens different [e.g., maximum BD-induced MFs older (8.0+/-1.0 [S.D.]x10(-6)) younger (17.8+/-6.1 x 10(-6)) observed 8 5 post-exposure, respectively], but burden was both groups. assess gender male rodents measured when are expected occur post-exposure. data demonstrated pattern susceptibility from high-level is mice>male mice>female rats>male Exposures caused minor significant response compared with controls (n=16/group; P=0.03). These results help explain part differing outcomes/interpretations earlier mutation studies BD-exposed rodents.