Various strategies for obtaining oxidative artificial hemoproteins with a catalytic oxidative activity: from "Hemoabzymes" to "Hemozymes"?

摘要: The design of artificial hemoproteins that could lead to new biocatalysts for selective oxidation reactions using clean oxidants such as O2 or H2O2 under ecocompatible conditions constitutes a really promising challenge wide range industrial applications. In vivo, are performed by heme-thiolate proteins, cytochromes P450, catalyze the drugs dioxygen in presence electrons delivered from NADPH cytochrome P450 reductase. Several strategies were used mimic these enzymes, associate synthetic metalloporphyrin derivatives protein is supposed induce selectivity catalyzed reaction. A first generation "hemoabzymes" was obtained non-covalent association hemes N-methyl-mesoporphyrin IX, Fe(III)-α3β-tetra-o-carboxyphenylporphyrin microperoxidase 8 with monoclonal antibodies raised against cofactors. antibody-metalloporphyrin complexes displayed peroxidase activity and some them regio-selective nitration phenols H2O2/NO2 stereo-selective sulphides H2O2. second "hemozymes", non-relevant proteins derivatives. used, most successful which, named "host-guest" strategy involved incorporation into easily affordable proteins. found be able perform efficiently stereoselective organic compounds alkenes KHSO5.