Characterization and cDNA-microarray expression analysis of 12-oxophytodienoate reductases reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response.

摘要: Summary 12-Oxophytodienoate reductases (OPRs) belong to a family of flavin-dependent oxidoreductases. With two new tomato isoforms reported here, three OPRs have now been characterized in both and Arabidopsis. Only one these (OPR3) participates directly the octadecanoid pathway for jasmonic acid biosynthesis, as only OPR3 reduces 9S,13S-stereoisomer 12-oxophytodienoic acid, biological precursor acid. The subcellular localization was analyzed protein activity were consistently found peroxisomes where they co-localize with enzymes β-oxidation which catalyze final steps formation is thus confined plastids and, contrast previous assumptions, does not involve cytosolic compartment. expression (Lycopersicon esculentum,Le) context defense-related genes using microarray comprising 233 cDNA probes. LeOPR3 be up-regulated after wounding induction kinetics resembling those other enzymes. In wound response proteins (e.g. proteinase inhibitors), accumulation transcripts more rapid transient wounded leaves, but hardly detectable unwounded, systemic leaves. Consistent data, OPDA JA accumulate locally systemically leaves plants. transcriptional activation high levels are, required defense gene tissues.