Effect of scatter factor on motility of epithelial cells and fibroblasts

摘要: The scatter factor is a protein released by fibroblasts that causes dispersal of epithelial cell colonies and disruption intercellular junctions, as well an alteration morphology with ruffling rapid extension movement pseudopodia. To find out if the has direct effect on migration, Boyden chamber assay was used to determine partially purified migration cells through 8 microns pore size filters. results showed mobility Madin-Darby canine kidney (MDCK) stimulated, usually maximal at 100 ng per ml, which equivalent 200 units activity in standard based arrangement cells. due chemotaxis chemokinesis. A keratinocyte line also sensitive were, lesser extent, BSCl monkey motility freshly isolated fibroblast lines, however, not significantly affected. confirm specificity paracrine role show this fibroblast-derived molecule can directly stimulate