Assessment of the use of LED phototherapy on bone defects grafted with hydroxyapatite on rats with iron-deficiency anemia and nonanemic: a Raman spectroscopy analysis

作者: Isabele Cardoso Vieira de Castro , Cristiane Becher Rosa , João Alves dos Reis Júnior , Luiz Gaudêncio Passos Moreira , Juliana S Aragão

DOI: 10.1007/S10103-014-1562-Z

关键词:

摘要: This study aimed to assess bone repair in defects grafted or not with hydroxyapatite (HA) on healthy and iron-deficiency anemia (IDA) rats submitted LED phototherapy (LED-PT) by Raman spectroscopy. The animals were divided eight groups five each: Clot; Clot + LED; IDA Graft; Graft LED. When appropriated, irradiation IR (λ850 ± 10 nm, 150 mW, CW, Φ = 0.5 cm2, 16 J/cm2, 15 days) was carried out. shifts: ∼960 [symmetric PO4 stretching (phosphate apatite)], ∼1,070 CO3 (B-type carbonate ∼1,454 cm−1 [CH2/CH3 bending organics (protein)] analyzed. mean peak values for ∼960, ∼1,070, nonsignificantly different anemic rats. group showed the lowest when compared irradiated (p ≤ 0.001; p 0.001). association of LED-PT HA-graft at results this indicated higher HA peaks as well a decrease level organic components graft are associated. In other hand, condition interfered incorporation only improved used.

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