Real-Time Measurements of Protein Affinities on Membrane Surfaces by Fluorescence Spectroscopy

作者: Finly Philip , Suzanne Scarlata

DOI: 10.1126/STKE.3502006PL5

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摘要: Signal transduction in cells involves transitory interactions between proteins and membranes different of the interacting species. These associations depend on strength local concentration. Because energy intensity fluorescence many probes are very sensitive to environment, measurements can report events, such as membrane binding protein association, real time. We describe methods monitor both vitro vivo by fluorescence.

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