DIAGNÓSTICO MOLECULAR DE LEPTOSPIROSE NO SANGUE DE CÃES NATURALMENTE INFECTADOS

摘要: ABSTRACT. Meira C.D., Wenceslau A.A., Carvalho F.S., Albuquerque, G.R. & Dias R.C. Molecular diagnosis of Leptospirosis in blood dogs naturally infected. [Diagnostico molecular de leptospirose no sangue caes naturalmente infectados]. Revista Brasileira Medicina Veterinaria, 33(1):7-11, 2011. Programa Pos-Graduacao em Ciencia Animal, Universidade Estadual Santa Cruz, Rodovia Ilheus-Itabuna, km 16, Ilheus, BA 45662-900, Brasil. E-mail: wenceslau@uesc.br is an acute and systemic infectious disease, which affects men animals caused by pathogenic spirochetes the genus Leptospira spp. The early important for adoption appropriate therapy prevention against installation critical symptomatologic forms. Polymerase Chain Reaction (PCR) being used diseases showing efficiency disease before development antibodies. aim this work was to evaluate PCR technique with primers G1 G2 that amplify a fragment 285 base pairs, specific region gene secY spp, on 200 samples from domestic stray determination risk factors associated Municipality Ilheus State Bahia. Nine (4.5%) presented positive results leptospirosis. There statistically significant difference between prevalence leptospirosis domiciled or (p= 1.0), sex 0.455), age (p=0.644), access street 0.113) presence and/or contact rodents 0.362).