Acquisition of embryogenic potential in carrot cell-suspension cultures.

摘要: Embryogenic suspension cultures of domesticated carrot (Daucus carota L.) are characterized by the presence proembryogenic masses (PEMs) from which somatic embryos develop under conditions low cell density in absence phytohormones. A culture system, referred to as starting cultures, was developed that allowed analysis emergence PEMs newly initiated hypocotyl-derived cultures. potential, reflected number FEMs present, slowly increased over a period six weeks. Addition excreted, high-molecular-weight, heat-labile factors an established embryogenic considerably accelerated acquisition potential Analysis [35S]methionine-labeled proteins excreted into medium revealed distinct changes concomitant with these pattern gene expression in-vitro translation total cellular mRNA different and subsequent separation products two-dimensional polyacrylamide gel electrophoresis demonstrated small abundant in-vitro-translation be present cells but absent nonembryogenic cells. Several other were explants, non-embryogenic embryos. Hybridization embryoregulated complementary-DNA sequence, Dc3, RNA extracted showed corresponding is expressed not