Ubiquitous human adeno-associated virus type 2 autonomously replicates in differentiating keratinocytes of a normal skin model.

摘要: Since its discovery in 1966, adeno-associated virus type 2 (AAV) has been described as a helper-dependent parvovirus. However, this study we demonstrate that AAV undergoes complete life cycle, devoid of helper viruses or genotoxic agents, the organotypic epithelial raft tissue culture system, model normal skin. progeny production directly correlated with differentiation, nondifferentiating keratinocytes were defective for activity. Large nuclear arrays particles approximately 26 nm (parvovirus size) observed granular layers epithelium by electron microscopy. Additionally, dosage-dependent histologic changes, some which might be interpreted cytopathology, induced AAV-infected tissues. These data suggest new biological AAV; is, is an epithelial-tropic autonomous parvovirus can alter squamous differentiation.