GC–MS profiling of the phytochemical constituents of the oleoresin from Copaifera langsdorffii Desf. and a preliminary in vivo evaluation of its antipsoriatic effect

摘要: Copaiba is the oleoresin (OR) obtained from Copaifera (Fabaceae), a neotropical tree which grows in Amazon regions. The balsam, constituted by an essential oil and resinous fraction used as folkloristic remedy treatment of several inflammatory diseases for its antioxidant antibacterial properties. Aim this work was (a) to carry out characterization GC-MS volatile nonvolatile constituents langsdorffii Desf. (OR); (b) investigate mechanism anti-inflammatory activity; (c) evaluate antipsoriatic effect after oral intake/topical application. (yield: 22.51%, w/w) shows: α-bergamotene (48.38%), α-himachalene (11.17%), β-selinene (5.00%) β-caryophyllene (5.47%). OR residue (77.49%, w/w), derivatization, showed main following compounds: copalic, abietic, daniellic, lambertinic, labd-7-en-15-oic, pimaric, isopimaric acids kaur16-en18-oic acid. Preincubation LPS-stimulated human THP-1 monocytes with increasing concentrations purified (OR-PF), containing diterpene acids, diterpenes sesquiterpenes, reduced release pro-inflammatory cytokines (IL-1β, IL-6, TNFα) dose-range 0.1-10 μM. In addition, cell culture system monocytes, 1 μM OR-PF counteracts LPS-driven NF-κB nuclear translocation. preliminary clinical trial three patients affected chronic psoriasis, treated intake or topical application OR, exhibited significant improvement typical signs disease, i.e. erythema, skin thickness, scaliness. conclusion, results work, beside extensive analytical chemical composition, provide strong evidences that activity related inhibition translocation, consequently proinflammatory secretion.