Serological differentiation between Echinococcus granulosus and E. multilocularis infections in man
作者： B. Gottstein , J. Eckert , H. Fey
DOI: 10.1007/BF00927876
关键词:
摘要: An enzyme-linked immunosorbent assay (ELISA) was adapted for the serological differential diagnosis of cystic or alveolar echinococcosis in man caused by Echinococcus granulosus E. multilocularis respectively. By affinity chromatography using rabbit anti hydatid fluid IgG coupled covalently to CNBr-Sepharose 4B a protein fraction (Em 1) containing shared antigens both parasites could be isolated from an extract metacestode tissue. From same source another antigen 2) with high degree specificity prepared immunosorption. Antigen Em 1 equally sensitive detection antibodies against and multilocularis, whereas 2 appeared more specific multilocularis. A correct achieved 95% 57 confirmed cases human simultaneous use fractions ELISA comparison their reactivities.
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