Amino acid and amino sugar determination by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate followed by high-performance liquid chromatography and fluorescence detection

作者: J Diaz , J Ll Lliberia , L Comellas , F Broto-Puig , None

DOI: 10.1016/0021-9673(95)00372-X

关键词:

摘要: A method for the derivatization of amino acids with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) use HPLC was tested. The reproducibility, linearity and limits detection are similar to those obtained other methods, such as o-phthalaldehyde 9-fluorenylmethoxycarbonyl methods. ease sample preparation possibility sugar resolution reasons why this chosen simultaneous acid analysis. need a previous step reduction studied it concluded that is not necessary.

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